I you mean macrovision, i think it's only for pressed DVD and you need a licence from macrovision. Other "copy protection" are to set copyright ON in your burning soft. But this does just produce a warning "this dvd is copyrighted" , no real protection. Anyway, macrovision is just from an old time, to disable dvd copy to vcr, and is easily defeat by small cheap device available everywhere. And in no case it disable DVD copy in a PC...
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as Macrovision is just a flag in the video stream I think it should be possible to activate it during the authoring phase but your authoring program has to be equipped for it (contact the place where you bought your authoring software, they'll be able to help you with it). Same goes for CSS encryption except there you prepare the disc in your authoring program but the actualy CSS encryption is only applied once you send the disc to replication (and if you're sending a DVD-R for replication you need to get the more expensive DVD-R for authoring version which also requires a different - and more expensive - burner). In the end you have to ask yourself if it's worth the effort because neither CSS or macrovision are particularly effective against anything but really casual (or clueless?) copy attempts.